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高分子科学（英文版）
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2026年7期
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Microenvironment Responsive Polymeric Nitric Oxide Releasing Micelles for Enhancing Eradication of Methicillin-resistant Staphylococcus aureus Biofilm
Microenvironment Responsive Polymeric Nitric Oxide Releasing Micelles for Enhancing Eradication of Methicillin-resistant Staphylococcus aureus Biofilm
摘要
Biofilm infections pose a severe threat to global public health owing to their persistent and recalcitrant nature.The physical barrier formed by the biofilm impedes the penetration of antimicrobial agents,leading to a significantly reduced efficacy of conventional antibiotics.Herein,we developed a polymeric micelle system that responds to the biofilm microenvironment to release nitric oxide(NO),which is capable of disrupting biofilms,thereby enhancing the bactericidal efficacy of antibiotics against embedded bacteria.The hydrophobic small-molecule NO donor was first conjugated to a diblock copolymer composed of N-hydroxyethyl acrylamide and N-acryloyl morpholine to yield an amphiphilic diblock copolymer.This amphiphilic copolymer then self-assembles into polymeric NO-releasing micelles(PNOM).Upon exposure to thiol-con-taining molecules in the reducing biofilm microenvironment,PNOM responsively released NO in a sustained manner over several days.In vitro studies have demonstrated that PNOM significantly potentiated the anti-biofilm efficacy of levofloxacin(Lev)against methicillin-resistant Staphy-lococcus aureus(MRSA).The combination of PNOM and Lev dispersed 85.3％of the biofilm biomass and eradicated 98.8％of the embedded bac-teria.Moreover,in a murine model of implant-associated MRSA biofilm infection,PNOM was validated to enhance the antibiofilm efficacy of Lev in vivo,achieving a bactericidal rate of 93.9％for MRSA biofilms and significantly alleviating inflammation.In summary,we designed a polymeric micelle system that triggers NO release in response to a thiol-rich biofilm microenvironment,thereby disrupting biofilm formation and enhanc-ing the antibiofilm effect of antibiotics against MRSA.This approach represents a promising therapeutic strategy for treating stubborn biofilm-as-sociated infections.
年，卷(期)：
2026, (7)
机标分类号：
S
S15
TH16
在线出版日期：
2026年9月9日
